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Lohr C, Grosche A, Reichenbach A, Hirnet D (2014) Purinergic neuron-glia interactions in sensory systems

Use separate syringes for each injection

The problem is usually reduced or eliminated by simply adding Tween 20 detergent to a final concentration of up to 0.1% to the beads, followed by resuspension and washing in buffer without the detergent