DMEM, High Glucose, GlutaPlus, no leucine Human Tumor Cell information

information

PROTOCOLS FOR THAWING THE CELLS AND SUBCULTURE A) Pre-coating of T25 flasks- Add 2ml each Universal Coating Solution  (UBP-01) into a T25 flask to cover the whole surface of the flask

transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long- term storage

Frozen Cells Protocol:

Hemocymeter calculation method:Cell number of 1 mm quadrel X 104(cell dilution) = actual cell number/ml

DMEM, High Glucose, GlutaPlus, no leucine Human Tumor Cell informationProduct Information Product Name Cat. No. Spec. DMEM, High Glucose, GlutaPlus, no leucine UBP4624 500 mL Product Description Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293,