Cooling Coil Hole size µm Percentage of

Hole size µm Percentage of open area 254 19 381 21 457 31 508 21

but can be autoclaved prior to the first use

The Bio-Gel P-4 polyacrylamide beads are used at the time of Perifusion chamber setup

Advanced Programmable Controller provides five-point temperature calibration capability

with a heating coil

Cooling Coil Hole size µm Percentage ofStainless steel cooling coils are used to lower the temperature of the digestive solution during the dilution phase. Cooling inactivates the collagenase to prevent healthy islets cells from being digested. This coil is used with a cold water bath or with the Ricordi Isolator Chiller. Individual coils are not sold sterile, but can be autoclaved prior to the first use. Biorep strongly suggests using a new coil for each isolation. Attempting to clean and