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The Co-N coordination bond between cobalt and DMB is, however, not strong, and the nucleotide base can dissociate depending on: (a) pH, (b) temperature, (c) protein environment, and (d) oxidation state of the Co-ion

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The diagnosis of vitamin B12 deficiency typically relies on low total B12 levels and elevated concentrations of homocysteine (Hcy), as well as levels of transcobalamin-bound B12 (holoTC) and methylmalonic acid (MMA), which serve as functional indicators of cobalamin insufficiency (Carmel and Sarrai, 2006)