CRISPR Mouse Genome 86K Knockout Library shRNA Constructs The CloneTracker XP barcode constructs

The CloneTracker XP barcode constructs are selectable by puromycin or hygromycin and a red fluorescent protein (RFP) marker

IGL) with sensitive and reproducible profiling across multiple sample types

Each kit provides enough reagents to measure this activity in 5-10 cell lines

Lentiviral vector for cloning and expressing cDNA with 2A self-cleaving peptides element so it expresses a single transcript that also expresses the selection marker

and reagents required for library preparation

CRISPR Mouse Genome 86K Knockout Library shRNA Constructs The CloneTracker XP barcode constructsCellecta's CRISPR Mouse Genome Wide Knockout Library was designed to be small enough to enable convenient, genome wide "dropout viability" screens. With approximately 86,000 constructs, the updated (Dec. 2023) mouse CRISPR library targets all mouse protein coding genes (~21,300). With 4 sgRNAs targeting each gene, nearly all of which have been designed using the criteria for effective guides described by Doench, et al. and containing the Cellecta