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For immunoblotting, the membranes were incubated with primary antibodies at 4 C overnight and then with secondary antibodies for 1 h at room temperature

Brand MD (2000) Uncoupling to survive

293 , E1580E1589 (2007)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 80.Sethumadhavan S, Silva M, Philbrook P, Nguyen T, Hatfield SM, Ohta A, et al

Ophthalmological disorders, including age-related macular degeneration (AMD), diabetic retinopathy (DR), and glaucoma, are major causes of blindness and visual impairment worldwide (Wong WL