CRISPR-Test Cas9 and dCas9 Activity Test Kits Type:CRISPRaTest dCas9-Activator Assay Kit (to test 5-15 cell lines) sgCopGFP Control (copGFP targeting) in

sgCopGFP Control (copGFP targeting) in pRSGT16-U6Tet-sg-CMV-TetR-RFP-Puro

Gene list: DECIPHER Gene List Mouse Module 2

These library pools have the barcode in the 3'-UTR compatible with oligo-dT cDNA synthesis protocols

Each kit contains 1 x 107 TU of either 5 or 10 individual pre-packaged lentiviral particles to label millions of cells

This Assay is similar to the CRISPRuTest Cas9 Fluorescent Reporter Assay in that both use the disappearance of a fluorescent marker to assess Cas9 activity

CRISPR-Test Cas9 and dCas9 Activity Test Kits Type:CRISPRaTest dCas9-Activator Assay Kit (to test 5-15 cell lines) sgCopGFP Control (copGFP targeting) inCellecta's CRISPR Functional Cas9 and dCas9 Activity Test Kits measure the functional activity of Cas9 nuclease, dCas9 repressor, or dCas9 activator by measuring loss of GFP in any mammalian cell line. 1. a. CRISPRuTest Functional Cas9 Activity Assay Kit (Catalog # CRUTEST) can be used to measure the Cas9 nuclease activity in any vertebrate cell system. The CRISPR system uses a target single guide RNA molecule (sgRNA) to target and cut a specific