CRISPR Inducible copGFP sgRNA Control Construct in Lentiviral Vector (no Cas9) TCR profiling The second option is a

The second option is a construct expressing Cas9-BFP fusion when doxycycline is present

The progeny from each cell population can be sampled after differently labeled populations are co-cultured or otherwise grown together

plasmid  Order Now >  Manual and Product Documentation Flyer: Cellecta InDOXible Tet-Activated Lentiviral cDNA Expression System

Typically used as a negative control for RNAi studies

PCR and NGS stages

CRISPR Inducible copGFP sgRNA Control Construct in Lentiviral Vector (no Cas9) TCR profiling The second option is aTet inducible sgRNA targeting the copGFP fluorescent protein in a lentiviral vector (no Cas9). The U6 promoter driving sgRNA expression is responsive to the tetracycline analog doxocycline. Usually used as a CRISPR negative control, but can be used as a positive control if cells have been engineered to express copGFP. Lentiviral copGFP expression construct is available separate from Cellecta. Catalog # Description Vector Type SGCTL COP pRSGT16