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Lysates were loaded onto NuPAGE 412% Bis-Tris acrylamide gels (1.0 mm, 10-well, Invitrogen), electrophoresed with MES running buffer and then transferred onto 0.45 m PVDF membrane by electroblot using a Mini Blot module (Invitrogen)

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Their complementary mechanisms (signaling amplification via BPC 157 + cell migration facilitation via TB 500) provide a scientific rationale for combined investigation