Human Genome-Wide CRISPRi Dual-sgRNA Library DriverMap Targeted RNA Sequencing 250 ug is enough for

250 ug is enough for ~12 10-cm2 plates to make ~1-2 x 10^7 TU

IGK and IGL chains are shown

a compatible  Universal Unique Dual Index (UDI) NGS Index Plate  must be purchased separately as a required component of the workflow

SVRTTATB-P Dox-On | No RFP Sensor All-in-One Dox-On Cloning Vector (pTMONBla-TRE-MCS-EFS-rtTA-2A-Blast)

top-ranking genes from the Cancer Genome Atlas

Human Genome-Wide CRISPRi Dual-sgRNA Library DriverMap Targeted RNA Sequencing 250 ug is enough forPooled library of ~105,000 dual sgRNA constructs targeting ~19,000 human protein coding genes. Each construct expresses two sgRNA targeting the same gene. Designed to work with deactivated spCas9 (dCas9) complexed with the KRAB repressor to inactivate or down regulate gene expression. Five CRISPRi dual sgRNA constructs target each gene. Designs based on Horlbeck, et al., eLife 2016; 5: e19760 DOI: 10. 7554). Manual and Product Documentation Manual: