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1 Introduction Pediatric low-grade glioma (pLGG, WHO grade 1 or 2) is the most common brain tumor class in children ( 2 Molecular landscape of pLGG Advances in sequencing technologies have aided in classifying the diverse spectrum of pLGG histopathology

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The principle is based on the different membrane permeability to the membrane-impermeable fluorescent DNA intercalating agent PI which stains membrane-compromised cells (red fluorescence) [40]