Collagel Hydrogel R Human Cells carefully remove any excess coating

carefully remove any excess coating solution and rinse the flask twice with 5ml of 1x PBS

Inspect to make sure the flask is at 90% confluence

accounting for 70-75% of the total pool

ENDOGrowth medium (EGM-2102) containing 5% serum and growth supplement is recommended for culture

The Cytoplasmic VWF/ factor VIII

Collagel Hydrogel R Human Cells carefully remove any excess coatingPrecautions and Disclaimer The handling of any organisms derived products has potential to be biologically hazardous. Proper precautions must be taken to avoid exposure. Always wear proper protective equipment (Gloves, safety glasses, etc.) when handling these materials. We recommend following the universal procedures for handling products of any organisms origin as the minimum precaution against contamination. All product offered by Universal