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Immunofluorescence staining was also performed as previousely described 44

doi: 10.1002/prp2.825 146 AryanHFarahaniRHChamanaraMElyasiSJaafariMRHaddadMet al

Ojeda MJ, Cereto-Massagu A, Valls C, Pujadas G, Pujadas G

In a study of 28 treatment-resistant BD patients, ECT showed a decrease in the pre-treatment elevated levels of MDA, a lipid peroxidation biomarker, in responders compared to non-responders [184]

The following primary antibodies were used: anti-UCHL3 (ab126621, Abcam), and anti-Actin (sc-47778, Santa Cruz)

The accumulated iron drives the expansion of mitochondrial reactive oxygen species (ROS) and lipid peroxidation through Fenton reactions, ultimately resulting in damage to DNA, lipids, and proteins 8,9