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[DOI] [PubMed] [Google Scholar] 143.Rodriguez-Sargent C., Cangiano J., Berros Cabn G., Marrero E., Martnez-Maldonado M

However, combinatorial regimens integrating these compounds with conventional chemotherapeutic agents not only potentiate natural product efficacy but also reverse chemoresistance via synergistic ROS-mediated mechanisms

R.SantiagoM.MachadoA.CanoJ

Cell culture and treatment Immortalized human keratinocytes (HaCaT cells), purchased from the China Center for Type Culture Collection, were cultivated in RMPI 1640 medium and complemented with 10% Fetal bovine serum (Gibco, USA), penicillin and streptomycin (NCM, China)

More importantly, the experiments of chemical inhibition and cKS were all carried out in RPMI -gln or RPMI Pgln medium containing ~5 times higher asparagine levels (378 M) than plasma (4080 M), suggesting that the extracellular asparagine could not compensate for the endogenous asparagine deficit in Pf caused by GS inhibition

Incorporation of multiple metals into a single nanoplatform further amplifies ROS generation and synergistic therapies like PDT, CDT, and PTT [79]