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In brief, 50-100 mg leaf samples were frozen in liquid nitrogen, and ground with 1 mL Extraction Buffer [50 mM EPPS buffer, pH 8.0, containing 1 mM EDTA, 10 mM DTT, 0.1% Triton X-100 [v/v], 0.5% polyvinylpyrrolidone, and 1x SigmaFAST Protease Inhibitor Cocktail, EDTA Free (Sigma)] on ice using 2 mL glass-to-glass homogenizer

doi: 10.1158/2326-6066.CIR-17-0316 212 ZhaoYLeeCKLinCHGassenRBXuXHuangZet al

Berjis: In the US, claims that are generally well-supported are usually in the structure or function territory, meaning those that support normal body function, rather than treating disease

Notably, Fe and Zn in these patients remain normal, suggesting that SLC39A14 deletion specifically affects Mn (Tuschl et al., 2016

Type C phospholipase A subclass of enzymes that cleave the polar head group of phosphoinositides between the glycerol and phosphate moieties

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