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doi: 10.1086/507543 198

Cell culture and reagents The human gastric adenocarcinoma cell lines SGC7901 (obtained from the Academy of Military Medical Science, Beijing, China) and the MDR variants, SGC7901/ADR and SGC7901/VCR (established and maintained in our laboratory), and AGS (obtained from the cell bank of Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Hyclone) and penicillin/streptomycin

Each vial should be inspected visually before each use for any signs of particulate matter, discoloration, cloudiness, or other changes in appearance

Published in Nature , this finding transformed the vocabulary of the field: compounds once called GHRP-like agonists were now understood as ghrelin mimetics or ghrelin receptor agonists, and the receptor gained an endogenous physiological anchor

This process involves unrestricted lipid peroxidation caused by the generation of reactive oxygen species (ROS) because of an abrupt increase in free Fe 2+ in the cytoplasm, all of which leads to subsequent membrane damage and iron-dependent cell death, now known as ferroptosis

PWL, paw withdrawal