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The relevance of the real-world evidence in research, clinical, and regulatory decision making

Mesenchymal chondroprogenitor cells differentiate into chondrocytes through cellular condensation processes, which are then surrounded by an abundant layer of extracellular matrix, including type II, IX, and XI collagens, which is the characteristics of cartilage (Mendler et al., 1989)

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The assay is based on reversed-phase high performance liquid chromatography with UV detection and simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide