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The fluorescent spot test is easiest to perform but is very unreliable for the detection of heterozygous women

TTN-AS1 is upregulated in LUAD tissues and associated with TNM stage, lymph node metastasis and poor postoperative prognosis

Recently, long-read sequencing has become possible by overcoming read-length limitations through two new technologies that have been developed for genome-wide studies: single molecule real-time (SMRT) sequencing from Pacific Biosciences (PacBio) and nanopore sequencing from Oxford Nanopore Technologies (ONT) [6, 25]