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The supernatant was then combined with 2.7 ml phosphate buffer (0.1 M, pH 7.4) and 0.2 ml 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) and incubated for 5 min

Targeting transforming growth factor- receptors in pulmonary hypertension

The percentage of SA--gal staining-positive cells was calculated in at least 3 random fields

Significant ANOVA/KruskalWallis test results were then followed with post-hoc testing, which underwent the two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli correction for multiple comparisons

It catalyzes Phase II detoxification reactions, neutralizes reactive oxygen species, and regenerates other antioxidants like vitamins C and E

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