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Glutathione is one of the major antioxidants produced by cells whose job is to neutralize free radicals and reactive oxygen species and maintain antioxidants obtained through diet, such as vitamins C and E

Clarified lysate was next applied to a Ni Sepharose 6 FF column (Cytiva) linked to an AKTA Avant150 FPLC system for immobilized metal affinity chromatography