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General Symptoms Constant tiredness or weakness Pale or yellowish skin Shortness of breath Heart palpitations Loss of appetite Weight loss Neurological symptoms of B12 deficiency Vitamin B12 plays a major role in nerve health

Samples were diluted in PBS, spun down and serum fractions were stored for later construct detection by sandwich ELISA

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But tobacco even smokeless also constitutes a causal factor in throat, mouth, kidney, bladder, pancreas, stomach, and cervical cancer