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The infant's blood-gas levels were monitored several times, and the findings suggested the presence of intractable metabolic acidosis

Hemopexin-dependent heme uptake via endocytosis regulates the Bach1 transcription repressor and heme oxygenase gene activation

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Department of Health and Human Services, ods.od.nih.gov/factsheets/VitaminB6-HealthProfessional/

(2015) 10:e0123597

The eluted protein was concentrated, and buffer exchanged into 50 mM NaH 2 PO 4 , 300 mM NaCl, 10 % v/v glycerol, pH 8.0 using Vivaspin 5 5000 Da MWCO spin columns (Cytiva)