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Cells were then resuspended in 500 L of free HBSS, strained through a 40 m cell strainer (BD Falcon), and analyzed using a flow cytometer (BD LSRFortessa Cell Analyzer, BD Biosciences) equipped with 488 nm laser for excitation

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Poirier, C., van Effenterre, D., Delord, B., Johannes, L

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The cells were seeded at a concentration of 5000 cells per cm 2 in a tissue culture treated flask and incubated at 37 C in a humidified incubator in the presence of 5% CO 2 (all subsequent incubations were subject to these conditions)

Nat Rev Immunol 9:778788 Pasparakis M (2012) Role of NF-kappaB in epithelial biology