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Only peptides that had summed S/N 160 across all 16 channels and had 50% isolation specificity in both the MS2 and MS3 spectra were considered for quantitation

doi:10.1097/prs.0000000000006327

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As the unconventional XBP1 mRNA splicing by IRE1 and the subsequent activation of the spliced XBP1 protein expression are the major mechanism of ER homeostasis regulation by IRE1 10 , we tested whether XBP1 plays a role in IRE1-mediated ferroptosis sensitization