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the procedure is called a tenotomy

Freezing creates ice crystals that can physically tear apart peptide chains

Materials and Methods Primary antibodies The following primary antibodies were used in this study: mouse anti-bovine serum albumin (B2901, 1: 1,000), rabbit anti-NOS1 (SAB4502010, 1: 500), and mouse anti--actin (A2228, 1: 10,000) antibodies from Sigma-Aldrich (St