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The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock

Extensive thiol profiling for assessment of intracellular redox status in cultured cells by HPLC-MS/MS

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Urine volume was recorded at the end of the collection period and aliquots were stored at 80 C until further analysis

Like in mouse sepsis, Nr1i3 and all CAR target genes investigated were downregulated in pig sepsis, although the downregulation was generally less pronounced in pigs than in mice, except for NR1I3 and the CYP genes ( Figures 10A, B )

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