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Once expressed, NOSII is constantly active and is not regulated by calcium, as are NOSI and NOSIII [49]

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The DESeq2 tool (R package, V1.30.1) was used to compare all transcripts of the read coverage tables, normalize counts between conditions and identify the differentially regulated transcriptions in gene level (Differentially Expressed Genes, DEGs)

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11, 12 weeks after injury, rats in the negative control group could not withdraw their paws from the hot plate within 12 s

Values were normalized with respect to the total protein on each sample determined by the BCA method (Sigma-Aldrich)