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Subsequently, these calculated values, along with the response and slope of the regression equation, were used to calculate the LOD and LOQ

Further elucidation of the exact role of O-GlcNAcylation in HD pathology will lead to a better understanding of the molecular etiology and pathophysiology of HD

doi: 10.1089/ars.2011.4391 37 GuoWLiKSunBXuDTongLYinHet al

Islet-specific CTL cloned from a type 1 diabetes patient cause beta-cell destruction after engraftment into HLA-A2 transgenic NOD/scid/IL2RG null mice

Denaturing immunoprecipitation Cells were lysed with 100 l of SDS-containing buffer (50 mM Tris pH 7.5, 1% SDS, 2.5 mM NEM) for 10 min on ice, scraped and SDS was diluted with 900 l dilution buffer (50 mM Tris pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP40 and freshly added 5 mM DTT, protease and phosphatase inhibitors)

FEBS Lett 584 (6) 12571262