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Proteins were detected on nitrocellulose membranes (Bio-Rad, #1620115) using the following primary antibodies: 6*His-tag (clone 4A4 or 2F12, source: antibody facility of the Helmholtz Center Munich, hybridoma supernatants were used at a 1:10 dilution) 75 , -TRAF6 (H-274, Santa Cruz Biotech., #sc-7221, used at 1:1000), -HA-tag (12CA5, Sigma-Aldrich, #11583816001, used at 0.4 g per mL), -IB (C-21, Santa Cruz Biotech., #sc-371, used at 1:500) and -Tubulin (B-5-1-2, Santa Cruz Biotech., #sc-23948, used at 1:500)

As shown in Figure 2A, we successively added BSA, Gly, GSH, NSE, and IL-6 to the reaction solution and detected it by electrochemical sensor

IGF-1 LR3 and Glucose Uptake in Adipocyte Models

Our genes determine the sequence of amino acids in a protein

The first step takes place in the liver where natural and estrogen-like substances are broken down

Hunter et al., 2003