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Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice

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11 In the mitochondria, carrier proteins such as oxoglutarate carrier (also known as SLC25A11) and dicarboxylate carrier (SLC25A10) play roles in the mitochondrial transport of GSH, 79 and are therefore also regulatory targets of ferroptosis

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The HlGST and HlGST2 fragments were amplified by PCR from plasmid clones using oligonucleotides, including HlGST T7 forward with HlGST RNAi reverse and HlGST T7 reverse with HlGST RNAi forward primers, and HlGST2 T7 forward with HlGST2 RNAi reverse and HlGST2 T7 reverse with HlGST2 RNAi forward primers, to attach the T7 promoter recognition sites on both forward and reverse ends

The sections were then incubated with FSB solution, and then soaked in lithium carbonate solution