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In severe MASLD, functional analysis revealed increases in fatty acid degradation and flavonoid biosynthesis, and decreases in pyrimidine metabolism, steroid biosynthesis, folate biosynthesis, and alanine, aspartate, glutamate metabolism

The proteasomal ubiquitin receptors Rpn10 and Rpn1 were both postulated to bind the NUB1 UBL domain, and Rpn10 was also assumed to bind to the NUB1 UBA domains and the FAT10 UBL2 domain, suggesting some form of competing interactions or order of events that led to a confusing model for FAT10 recruitment 35,40,42

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