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The fastq reads were processed with Kneaddata, which integrated FastQC [30], Trimmomatic [31] and Bowtie2 [32], allowing quality control and decontamination via alignment to the Rhode Island red laying hen genome ( as the assembled Lohmann Brown genome was not available at the time of writing

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Distinguishing the pathology of these acute events from that of the underlying disease is difficult because patients experiencing an exacerbation are usually too ill to study

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