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Because it modulates cellular NAD+ availability and mitochondrial function, some protocols administer it in the morning to align peak NNMT inhibition with waking metabolic activity

doi: 10.1038/nprot.2012.024

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5-Amino-1MQ was first described in a widely cited 2018 study that linked NNMT inhibition to a reduction in fat mass in obese mice without any change in food intake

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It has also been reported that 5-amino-1MQ treatment requires an acclimation period, with some subjects reporting decreased exercise tolerance with cardiovascular exercise