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4.1 Copper overload triggers apoptosis Apoptosis, a type of programmed cell death, is marked by substantial alterations in cell morphology and the activation of specific caspase and mitochondrial control pathways (130)

Vergote I, Armstrong D, Scambia G, Teneriello M, Sehouli J, Schweizer C, et al

ROS induce inflammation by increasing the production of pro-inflammatory cytokines, especially IL-1, IL-8, and TNF- (176, 177)

AGEs/RAGE stimulation activates immune cells, resulting in NADPH oxidase (NOX) overexpression, which is a primary cytoplasmic source of free radicals and pro-inflammatory factors such as tumor necrosis factor- (TNF-), interleukin 1 (IL-1) and interleukin 6 (IL-6) 5,6,7,8

The qPCR primers are listed in Supplementary Table 1

Passive skin penetration enhancement and its quantification in vitro