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The slides were incubated in Picrosirius Red solution (Abcam, ab150681) for 1 h and then drained on absorbent paper

The effect of systemic inflammation on human brain barrier function

The cell viability (%) is expressed as the percentage of (OD test OD blank )/(OD control OD blank ), where OD test is the optical density of the cells exposed to MnO 2 nanocomplex or nanoprobe, OD control is the optical density of the control sample and OD blank is the optical density of the wells without cells

Minerals: Due to alcohol-related vomiting, diarrhea, GI bleeding and overall nutrient problems, low mineral levels are common in chronic drinking

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