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Cells were fixed with 4% formaldehyde and 5000 events were analyzed on a BD Accuri C6 Flow Cytometer

These factors include a limitation of nutrients, a lack of oxygen, competition for scarce resources, acidity, and saturation with immunosuppressive factors (73, 74)

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Tonnus W, Meyer C, Steinebach C, Belavgeni A, von Massenhausen A, Gonzalez NZ, et al