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This motion can be seen by the increase in the inter-subunit F426F426 distance from 14 (substrate-bound) to 18 (occluded) and then to 29 (outward-facing)

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Nevertheless, whether a specific mutation in a given gene exerts a particular biological effect, rather than functioning simply as tumor-promoting or tumor-suppressing, remains insufficiently explored

To validate these computational findings, laboratory experiments using fluorescence binding and displacement assays were conducted to determine the actual binding affinities of the curcumin derivatives