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However, GSAAT(C190S) accumulated to a lesser extent than any of the other mutants, albeit still more than that expressed in the gsa2 mutant

(2019) CannabidiolFrom plant to human body: a promising bioactive molecule with multi-target effects in cancer

R., Grantham, P

Then, a second PCR was performed to validate the correct genomic integration of the transgene (degron sequence) using primers over 1 kb upstream and downstream the homology arms (outside the region corresponding to the targeting vector) and by using a Phusion Plus DNA Polymerase (Thermo Fisher, #F630S)

[DOI] [PubMed] [Google Scholar] 395.Diao YY, Liu XY, Toh GW, Shi L and Zi J, Adv

High Anion Gap Metabolic Acidosis. StatPearls, StatPearls Publishing, 11 August 2021