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If there is remaining tissue work to do, the second cycle is structured with the results of the first cycle in mind

Mechanical force induces phosphorylation-mediated signaling that underlies tissue response and robustness in Xenopus embryos

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Handling Precautions: Before mixing and drawing up doses, wash hands and swab the vial stopper with alcohol to maintain sterility

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Response is tracked by clinical assessment and photography, not by guesswork