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For instance, the National Medical Products Administration (NMPA) recognized butylphthalide as a therapeutic agent for ischemic stroke in 2005 (Zou et al., 2018)

The day of the experiment, cells were treated with test compounds for the indicated times, then harvested by trypsinization, resuspended in 500 L Hanks Balanced Salt Solution (HBSS, Gibco, Invitrogen Corp., Carlsbad, Calif.) containing either H 2 DCFDA (25 M), C11-BODIPY(581/591) (2 M) or MitoSOX (5 M) (all from Molecular Probes, Invitrogen) and incubated for 10 minutes at 37 C

Authorea Preprints Zomkowski AD, Santos AR, Rodrigues AL (2006) Putrescine produces antidepressant-like effects in the forced swimming test and in the tail suspension test in mice