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With the specific guidelines above, you should be able to remove iron bacteria from your water effectively.

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Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

Extracts were clarified by centrifugation and filtration through a 0.45 m pore Spin-X filter (Corning), and the total protein concentration was determined by BCA assay

Kong et al., 2023)

doi: 10.1016/j.neuint.2012.01.019 206 ParkerS