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Association of biochemical B 12 deficiency with metformin therapy and vitamin B 12 supplements: the National Health and Nutrition Examination Survey, 19992006

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EBC-1 cells were then seeded at a density of 2105 cells/cm2 in 35-mm, 60-mm or 100-mm dishes in serum-free MCDB-104GK medium supplemented with penicillin, streptomycin and neomycin and incubated further for 24 h as described above

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