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glabra and has pharmacological activities, including anti-oxidant, anti-inflammatory, and anti-cancer (Shibata et al., 1987)

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doi: 10.1016/j.biomaterials.2020.120153 87 MuzemboBAMbendiNCNgatuNRSuzukiTWadaKIkedaS

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Ni-NTA resin-bound proteins were washed six times by centrifugation at 3,000 rpm for 5 min each at 4C using BF supplemented with 10 mM imidazole