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8 The age of onset is usually after puberty and before the age of 40, peaking in the second and third decades of life

For mitochondrial staining, protoplasts were isolated from rosette leaves of 2-week old transformants that expressed OKI13xYPet driven under OKI1 native promoter in oki1 background using an enzyme solution (400 mM mannitol, 20 mM MES, 20 mM KCl, 10 mM CaCl2, 10 g/ml BSA, 15 mg/ml cellulase [PhytoTechnology Laboratories], 3 mg/ml pectolyase [Sigma-Aldrich])

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Three recent studies used ultra-high field strength (7T) MRS, as its greater spectral resolution and signal-to-noise ratio help to distinguish between these two metabolites

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