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In mammals, the PPP occurs exclusively in the cytoplasm

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For saturation binding assays, cell lysates (100 L, 25 g of protein for HEK-293 cells and 1020 g of protein for INS-1E cells, quantified by the Bradford Assay) were incubated with the indicated concentrations of [ 3 H]RX821002 (90 min, room temperature)

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(2019) Arthritis Research & Therapy |

On the other hand, beneficial metabolites such as butyrate can activate cellular mechanisms for protein clearance: butyrate readily crosses the BBB and can inhibit histone deacetylases, thereby activating gene expression programs that enhance autophagy and reduce toxic protein aggregates [55]