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Cathepsin B activity was then assayed in final buffer conditions of 0.04 ng l 1 cathepsin B, 40 M Z -Phe-Arg-AMC (Sigma), 40 mM citrate phosphate (pH 5.5), 1 mM EDTA, 100 mM NaCl, 5 mM DTT and 0.01% Brij at 37 C

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There are many examples of viral infections that display the importance of the native disulfide-thiol balance, where a specific disulfide-thiol balance is required for many different purposes, such as maintaining reactivity, becoming destabilized, aggregating or enabling viral membrane fusion with the target cell [6, 45]