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The 10dilution buffer (10% Triton x-100, 1 M NaCl, 1% Na-Deoxycholate, 5% N-Lauroylsarcosine, 5 mM EGTA) was added to the lysate, samples were centrifuged at maximum speed at 4 C for 20 min, and the following antibodies were added: -GFP (Santa Cruz, sc-9996, 5 g per sample), -BRD4 (Fortis, A700-004, 5 g per sample), -ASXL1 (made inhouse, 40 L anti-sera per sample), or -BAP1 (made inhouse, 40 L anti-sera per sample)

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To clone vanAB from ADP1 genome it was amplified with primers VanAB_F_BB and VanAB_R_BB

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Protease activity enhances production of thymic stromal lymphopoietin and basophil accumulation in flaky tail mice