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For optimal results, adherence to the recommended six-session protocol, proper post-treatment care, and lifestyle considerations such as sun protection and hydration are essential

Stereotaxic injection Mice were anesthetized with isoflurane, and then fixed into a stereotaxic instrument (ZS-FD Zhongshi Dichuan)

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The compound did not appear to affect food intake, behavior, or general health status in treated animals

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Zootechnical, comparative gel-based proteomics and sensory analyses showed that EDTA supplementation did not affect fish growth, muscle metabolism and fish quality as edible food, respectively, in both species, unless higher concentrations of EDTA were used with negative effects on fish growth performance and feed efficiency