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The next day, the wells were blocked for 1 h (4% bovine serum albumin (BSA), 5% sucrose in phosphate-buffered saline (PBS)), and then 100 L of sample was added and incubated for 12 h

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Studies have confirmed (121, 122) that p53 can induce ferroptosis not only by inhibiting SLC7A11 activity but also by increasing CDKN1A expression or decreasing DPP4 activity, suggesting the dual regulatory role of p53 in the regulation of ferroptosis

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