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The specific kinases involved in occludin phosphorylation remain elusive

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Cells were grown in LB or TB full media at 37 C, 130 rpm to an optical density (OD) of 0.60.8 before protein expression was induced by adding 200400 M of isopropyl--D-thiogalactopyranoside (IPTG, Carl Roth GmbH, cat no